The Court of Arbitration for Sport upheld UEFA's ban on Russian teams taking part in its competitions while CAS deliberates on the case.
Football governing bodies FIFA and UEFA had decided together that all Russian teams, whether national or club sides, be suspended from participation in FIFA and UEFA competitions until further notice after Moscow's invasion of Ukraine.
The Russian Football Union appealed to CAS but the president of the Appeals Arbitration Division denied a request to lift the ban until CAS takes a final decision.
The decision meant that Spartak Moscow could not play their Europa League match against club RB Leipzig, allowing the German club to advance to the quarter-finals.
The ruling only applies to UEFA competitions, with Russia still looking to overturn a FIFA ban that would allow them to take part in World Cup qualifying playoff matches scheduled for the end of this month.