The board of the Uluru-Kata Tjuta National Park voted unanimously to end the climb because of indigenous sensitivities.
The giant red monolith in the Northern Territory is a sacred site for Aboriginal Australians.
Local people have long asked visitors not to climb the outcrop, which was known for many years as Ayers Rock.
Signs at the start of the climb ask people to abstain from going up in respect to the traditional law of the Anangu Aboriginal people, the custodians of the land.
