It began at 15:00 BST on Friday and will end on Monday at at 23:59 BST.
The "show of solidarity against online abuse" hopes to encourage companies to take a stronger stance against racist and sexist abuse by users.
Rugby union, cricket and rugby league have also joined the protest.
The Premier League released a statement prior to the four-day silence, saying it would not stop challenging companies "until discriminatory online abuse is removed from our game and wider society".