Craig Douglas and Dylan Rigby made their pleas at Birmingham Magistrates' Court on Friday.
The pair were charged on 16 September with promoting a lottery and advertising unlawful gambling.
The case has been adjourned until 6 February 2017, when the court will hold a hearing expected to last a week.
Mr Douglas is a prominent gamer and has a YouTube channel under the pseudonym Nepenthez which has more than 1.3 million subscribers.
