Sanzaar has announced the May 5 match would switch from the regular Allianz Stadium in Sydney to Brookvale Oval, home of the NRL's Manly Sea Eagles.
The Waratahs requested the change due to the possibility Allianz Stadium will host the A-League grand final that day.
The kick-off time has also changed.
Scheduled for a mid-afternoon kickoff, it has moved to 7.45pm.
The changes was carried out in consultation with the Blues.
SANZAAR CEO Andy Marinos said, "Sydney's busy sporting calendar has unfortunately necessitated this move but the Waratahs have made a sensible decision in taking the match into one of its rugby strongholds in the Northern Beaches. The change of kick-off time is sympathetic to the requirements of grass roots rugby and should also ensure a healthy turn out at the Waratahs versus Blues match later in the day."