The video, uploaded to YouTube, encourages girls to share a photo of themselves holding up a sign with what they want for the future, using the hashtag #WhatIReallyReallyWant.
It is part of the Project Everyone campaign, a United Nations-supported coalition of independent and corporate groups pushing a series of UN global goals including education, gender and wage equality and an end to violence against women.
The video, launched as part of Project Everyone's The Global Goals website, features international performers dancing in front of signs and murals spelling out some of the goals.
Stars featured include British girl band M.O., Taylor Hatala from Canada, Larsen Thompson from the US, Gigi Lamayne and Monoea from South Africa, Seyi Shay from Nigeria and Bollywood actress Jacqueline Fernandez from Sri Lanka.
"This is about modern day girl power," said the video's director MJ Delaney.
"The Spice Girls were about a group of different women joining together and being stronger through that bond.
"These differences are what we want to celebrate in this film, while showing there are some universal things that all girls, everywhere, really, really want."
Spice girls Victoria Beckham (Posh Spice), Mel C (Sporty Spice) and Emma Bunton (Baby Spice) shared the video on Twitter.
"Twenty years on — Girl Power being used to empower a new generation. So proud of The Global Goals," Beckham said.
Mel C said she was "Flattered and honoured that our crazy song is being used so beautifully."
An association with global cinema advertising association SAWA will see the video run in movie theatres internationally.
(The new version of Wannabe cast features an international cast)