Customers turned to Amazon and other online platforms during the COVID crisis, leading to record profits for the US giant.
But Amazon's breakneck growth is beginning to level as customers start to return to bricks and mortar shops.
Revenue climbed 27% to £113bn (£80bn) in its second quarter, but this missed analyst expectations.
Amazon shares fell 5% in after-hours trade.
Earlier in the COVID pandemic Amazon posted record profits, signed up more than 200 million customers to its Prime service, and recruited more than 500,000 workers to keep up with surging demand.
But vaccine rollouts and easing of restrictions have led some consumers to venture out to stores, while also using click-and-collect services, in competition with the speedy delivery of online orders provided by Amazon and its peers.
Amazon's net sales rose to $113.08bn in the second quarter to 30 June from $88.91bn in the same period a year earlier. Analysts on average had expected $115.20bn.
The world's biggest online retailer had moved its annual marketing bonanza, Prime Day, to June this year, hoping to sell more goods before shoppers went on holiday.
It said the event was the biggest two-day sales period ever for merchants on its platform.
But in the second quarter analysts have seen signs of slowing demand.
North America, Amazon's largest market, saw sales increase 22% in the second quarter, versus 43% in the same period a year earlier.
But profit rose 48% to $7.8bn, the second-largest quarterly result Amazon has ever announced.
The retail giant's founder Jeff Bezos stepped down as chief executive on 5 July, becoming executive chair of Amazon's board.
Andy Jassy took on role, after previously being in charge of Amazon's cloud computing division, Amazon Web Services (AWS).
In the second quarter AWS was a bright spot, seeing revenue rise by 37% to $14.8bn.
Mr Jassy said: "Over the past 18 months, our consumer business has been called on to deliver an unprecedented number of items, including PPE [personal protective equipment], food, and other products that helped communities around the world cope with the difficult circumstances of the pandemic.
"At the same time, AWS has helped so many businesses and governments maintain business continuity, and we've seen AWS growth reaccelerate as more companies bring forward plans to transform their businesses and move to the cloud."
Amazon's are the latest results from Big Tech this week. Apple, Microsoft and Google's parent firm Alphabet all reported soaring profits as the COVID pandemic continues. Facebook, however, said that it expects revenue growth to slow.