The Safety Check, first introduced in October 2014, allows Facebook users to spread the word that they are safe in the wake of a natural disaster or a crisis, and allows searches for those who might be in the affected area.
"Waking up this morning, I was horrified to hear about the shooting in Orlando," Facebook chief executive Mark Zuckerberg said on his official account.
"My thoughts and prayers are with the victims, their families and the LGBT community."