Uefa says its disciplinary panel will judge the charge of "improper conduct'' on Thursday (Friday NZT).
Simeone was fined €20,000 (NZ$33,000) by UEFA for making an obscene 'cojones' gesture after Atletico took the lead in a 2-0 win over Juventus in the first leg. Ronaldo's hat trick in the 3-0 win in the return leg in Turin last week sent Juventus to the quarterfinals.
Ronaldo celebrated at the final whistle by mimicking Simeone's gesture.