Hadfield said the second person shot had minor injuries and was hospitalized.
The shooting suspect remained on the coach bus for hours while tactical teams and negotiators came to the scene on Las Vegas Boulevard, which was closed between Flamingo Road and Tropicana Boulevard.
Around 3:20 p.m. (6:20 p.m. ET), the man surrendered. Police said he had a handgun.
No one else was on the bus, a white double-decker with advertising wrapping on the windows that makes it difficult to see inside.
The incident is not related to terrorism, Hadfield said. There also is no evidence that the incident is connected to a robbery earlier Saturday at the Bellagio.
Hadfield said he had no information about the shooting suspect or the victims.
Southbound lanes of Las Vegas Boulevard will remain closed during the investigation. Northbound lanes have reopened, police tweeted.